Cell Doubling Time Calculator

Find cell-culture doubling time from two counts.

Doubling time 16

Formula: doubling = duration × ln(2) ÷ ln(N/N₀)

Step-by-step with your numbers:
1. Values used:
2. Time between counts = 48
3. Initial count = 100,000
4. Final count = 800,000
5.
6. Doubling time = 16
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How long your cells take to double in culture.

The math behind it

doubling time = duration × ln(2) ÷ ln(final ÷ initial).

Worked example

100k → 800k in 48 h → 16 h doubling.

FAQ

Slow growth?

Check confluency, medium freshness and passage number.