Biology

Cell Dilution Calculator

Find how much stock cell suspension to dilute.

Calculated Output

Stock volume needed (mL) 2

Medium to add (mL) 8

Formula: C₁V₁ = C₂V₂ → V₁ = C₂V₂ ÷ C₁

Step-by-Step Breakdown

1. Values used:

2. Stock concentration = 5,000,000 cells/mL

3. Desired concentration = 1,000,000 cells/mL

4. Final volume = 10 mL

6. Stock volume needed = 2mL

7. Medium to add = 8mL

Did this calculator solve your problem today?

The math behind it

C₁V₁ = C₂V₂, so V₁ = C₂ × V₂ ÷ C₁. Add medium up to the final volume.

5M/mL to 1M/mL in 10 mL → 2 mL stock + 8 mL medium.

Counting cells?

Use a hemocytometer or automated counter to find the stock concentration.