Biology

DNA Concentration Calculator

Find DNA concentration from an A260 reading.

Calculated Output

DNA concentration (ng/µL) 25

Formula: conc = A260 × factor × dilution

Step-by-Step Breakdown

1. Values used:

2. A260 absorbance = 0.5

3. Dilution factor = 1

4. Conversion factor = 50 ng/µL per A260

6. DNA concentration = A260 absorbance x Dilution factor x Conversion factor = 0.5 x 1 x 50 = 25ng/µL

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The math behind it

conc = A260 × 50 × dilution (50 ng/µL per A260 unit for dsDNA; use 40 for RNA, 33 for ssDNA).

A260 0.5, dilution 1 → 25 ng/µL.

Purity?

A260/A280 ≈ 1.8 indicates pure DNA.