Enzyme Activity Calculator

Find enzyme activity in units.

Product formed (µmol)

Time

Enzyme volume (mL)

Activity (U) 3

Activity (U/mL) 3

Formula: U = µmol product ÷ time (min)

Step-by-step with your numbers:

1. Values used:

2. Product formed = 30 µmol

3. Time = 10

4. Enzyme volume = 1 mL

6. Activity = Product formed / Time = 30 / 10 = 3U

7. Activity = Product formed / Time = 30 / 10 = 3U/mL

Did we solve your problem today?

One enzyme unit (U) converts one micromole of substrate per minute.

The math behind it

activity (U) = µmol of product ÷ time (min); divide by enzyme volume for U/mL.

30 µmol in 10 min → 3 U (3 U/mL in 1 mL).

Specific activity?

Activity per mg of protein (U/mg).